Staphylococcus pseudintermedius can also cause canine pyoderma, but no exfoliative toxins or similar toxins have been reported. PCR with degenerate primers targeted to the conserved regions in ETA, ETB, and ETD from S. aureus and SHETB from S. hyicus, and subsequent chromosome walking identified a novel gene, designated as exi (exfoliative toxin of pseudintermedius) in S. pseudintermedius Diagnoses of Staphylococcus pseudintermedius have traditionally been made using cytology, plating, and biochemical tests. More recently, molecular technologies like MALDI-TOF, DNA hybridization and PCR have become preferred over biochemical tests for their more rapid and accurate identifications The Staphylococcus intermedius Group includes S. intermedius, S. pseudintermedius and S. delphini, coagulase-positive bacteria commonly isolated from animals. The identification of organisms belonging to this group is presently carried out usin
Staphylococcus intermedius group is a complex of organisms including Staphylococcus intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. The most commonly isolated member of the complex is S. pseudintermedius, which can be normal flora, found on the skin, nose and mucosal surfaces of dogs. It has been found to colonize other animals, such as pigeons, minks, horses, raccoons and goats. It is also the leading cause of skin and soft tissue infections, also known as pyoderma We aimed to: i) determine the phenotypic resistance profile of methicillin resistant Staphylococcus pseudintermedius (MRSP) isolated from infected dogs in three different veterinary hospitals in Buenos Aires, Argentina; ii) identify the SCCmec elements and resistance genes; and iii) analyze the clonal relationship between isolates and in regard of dominant lineages found in the world
Bacteria in the Staphylococcus intermedius group, including Staphylococcus pseudintermedius, often encode mecA-mediated methicillin resistance. Reliable detection of this phenotype for proper treatment and infection control decisions requires that these coagulase-positive staphylococci are accurately identified and specifically that they are not misidentified as S. aureus Coagulase activity is a key characteristic of Staphylococcus pseudintermedius; however, no coagulase gene or associated protein has been studied to characterize this activity. We report a recombinant protein sharing 40% similarity to Staphylococcus aureus coagulase produced from a putative S. pseudintermedius coagulase gene
Staphylococcus pseudintermedius is a major bacterial species associated with canine pyoderma and otitis. Fusidic acid is used to treat skin infections caused by Gram‐positive bacteria. The incidence of resistance to fusidic acid in S. pseudintermedius has importance in terms of limiting treatment options for bacterial infections We report the first diagnostic test for the identification of Staphylococcus pseudintermedius involving a simple PCR-restriction fragment length polymorphism approach. The method allows discrimination of S. pseudintermedius from the closely related members of the Staphylococcus intermedius group and other important staphylococcal pathogens of. Staphylococcus is a genus of Gram-positive bacteria comprising more than 40 different species encompassing the coagulase-negative staphylococci (CoNS), such as Staphylococcus epidermidis and Staphylococcus haemolyticus, and coagulase-positive staphylococci, such as Staphylococcus aureus and Staphylococcus pseudintermedius. Staphylococci form part of the normal flora of human and animal skin and mucous membranes and are commonly associated with opportunistic infections Biochemical Test and Identification of Staphylococcus epidermidis. Last updated: August 15, 2019 by Sagar Aryal. Basic Characteristics. Properties (Staphylococcus epidermidis) Capsule. Mostly Capsulated. Catalase. Positive (+ve) Citrate
Staphylococcus intermedius was first described in 1976, 1 but during the past few years there has been confusion about its classification. In 2005 a novel staphylococcal species, S. pseudintermedius, was described. 2 Isolates formerly identified as S. intermedius by phenotypic characteristics were then reclassified based on molecular techniques Frequently Asked Questions About Methicillin-Resistant Staphylococcus Pseudintermedius (MRSP) in Dogs & Cats What is Staphylococcus Pseudintermedius (S. Pseudintermedius)? S. pseudintermedius is a bacterium commonly found on the skin, mouth, nose, or in the gastrointestinal tract of ~50% of dogs and cats. It typically causes no problems at all but it can cause opportunistic infections. This [ Staphylococcus pseudintermedius is a common commensal organism of canines, but is also one of the most common causes of opportunistic infections [1-3].Recently, methicillin resistant S. pseudintermedius (MRSP) has emerged and disseminated internationally [4, 5], with 2 major sequence types (ST68 and ST71) representing the variety of clinical infections in most regions  The Impact of Accurate Staphylococcus pseudintermedius Identification on Appropriate Antimicrobial Susceptibility Testing. Limbago BM(1). Author information: (1)Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA BBL7@cdc.gov
Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs Keita Iyori, Junzo Hisatsune, Tetsuji Kawakami, Sanae Shibata, Nobuo Murayama, Kaori Ide, Masahiko Nagata, Tsuneo Fukata, Toshiroh Iwasaki, Kenshiro Oshima, Masahira Hattori, Motoyuki Sugai. Bacterial strains. Altogether, 59 strains previously identified as S. intermedius or as a couple S. intermedius/S. pseudintermedius (Table 1) deposited at the department of CNCTC and NRL for Staphylococci were identified with standard phenotypic methods that include production of tube coagulase, heat-stable nuclease, acetoin, pyrrolidonyl arylamidase, β-galactosidase, α- and β-hemolysins.
Staphylococcus pseudintermedius is a member of the Staphylococcus intermedius group (SIG) comprising representatives with similar phenotypic characteristics. The purpose of this study was to optimise a method for accurate differentiation of S. pseudintermedius in clinica Of the 116 total bacterial isolates obtained, the majority belonged to Gram-positive species, and the most common organism identified through biochemical and molecular methods was Staphylococcus pseudintermedius. Additionally, identification of a Staphylococcus schleiferi subspecies coagulans isolate was confirmed by molecular methods. All.
Background. Staphylococcus pseudintermedius is a major bacterial species associated with canine pyoderma and otitis. Fusidic acid is used to treat skin infections caused by Gram‐positive bacteria. The incidence of resistance to fusidic acid in S. pseudintermedius has importance in terms of limiting treatment options for bacterial infections.. Hypothesis/objective Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderm
Identification of vaccine candidate antigens of Staphylococcus pseudintermedius by whole proteome characterization and serological proteomic analyses. Natacha Couto, Joana Martins, Ana Mafalda Lourenço, Constança Pomba, Ana Varela Coelho * * Corresponding author for this work Wasit province, Iraq; and bacteriological identification of Staphylococcus species was performed. Identification of S. pseudintermedius was evaluated by biochemical tests, API staph, the Vitek®2 compact and detection of some genes. In general, out of 84 samples, 63 samples (75%) were positive for Staphylococcus species Staphylococcus pseudintermedius is a pathogenic bacterium of concern within the veterinary sector and is involved in numerous infections in canines, including topical infections such as canine pyoderma and otitis externa, as well as systemic infections within the urinary, respiratory and reproductive tract. The high prevalence of methicillin-resistant Staphylococcus pseudintermedius (MRSP. Reflection paper on meticillin-resistant Staphylococcus pseudintermedius. Agreed by SAGAM (Scientific Advisory Group on Antimicrobials) 2 June 2010 : Adoption CVMP for release for consultation ; 15 September 2010 . Methods used for identification of S. pseudintermedius. Staphylococcus pseudintermedius is a Gram- and coagulase-positive bacterium, which belongs to the Staphylococcus intermedius group (SIG), a group which consists of S. inter-medius, S. pseudintermedius, and S. delphini. Staphylococ-cus pseudintermedius naturally colonizes the skin, coat, nose, mouth, and anus of dogs, but can also become a
Staphylococcus pseudintermedius, one of the major pathogens involved in canine infections, can be difficult to identify to the species level. 1,3,8 In particular, separation of S. pseudintermedius from the other members of the Staphylococcus intermedius group (SIG; i.e., Staphylococcus intermedius and Staphylococcus delphini) can be challenging. 1,3,8 Members of the SIG share a high average. Staphylococcus pseudintermedius is a coagulase positive bacterium and found on the mucous membranes of approximately 90% of healthy dogs. This organism is an opportunistic pathogen 1.1 Taxonomy, basics biochemical characteristics and identification of Staphylococcus Abstract. Staphylococcus pseudintermedius is an important opportunistic pathogen of companion animals, especially dogs. Since 2006 there has been a significant emergence of methicillin-resistant S. pseudintermedius (MRSP) mainly due to clonal spread.This article reviews research on MRSP with a focus on occurrence, methods used for identification, risk factors for colonization and infection. There is a sudden emergence of meticillin-resistant Staphylococcus pseudintermedius (MRSP) in dogs and cats mainly due to clonal spread. Due to the multiresistant characteristics of these bacteria they constitute a new prominent risk to animal health. Although most infection could be controlled withou
. This strain demonstrates the expected synergistic beta-hemolysis with Listeria monocytogenes in the CAMP test (FDA BAM) laser desorption/ionisation time-of-flight identification technique is leading to more specific identification of previously unrecognised organisms. Background Staphylococcus pseudintermedius is a coag-ulase and DNase-positive Staphylococcus that is a commensal and pathogen affecting animals, particularly dogs, but rarely humans Staphylococcus pseudintermedius is a major opportunistic canine pathogen that has been associated with an increasing number of human infections. As an opportunist, S. pseudintermedius causes a wide range of infections, predominantly of skin and soft tissue but also device/implant related infections and systemic infections such as bacteremia
. as a novel species in 2005 (Devriese . et al., 2005), methicillin-resistant. (MRSP) was reported with increased frequency in the following year (van Duijkeren . et al., 2011). The bacterium is both a commensal on dogs, and a pathogen causing canine pyoderma and woun Diversity of Staphylococcus species strains based on partial kat (catalase) gene sequences and design of a PCR-Restriction Fragment Length Polymorphism assay for identification and differentiation of coagulase-positive species (S. aureus, S. delphini, S. hyicus, S. intermedius, S. pseudintermedius, and S. schleiferi subsp. coagulans) Characterization of Protein A and Sortase A in Staphylococcus pseudintermedius: Potential Targets for Novel Therapeutic Approaches. I have examined the final electronic copy of this S. pseudintermedius. to identify novel vaccine targets. In this study, a 3-D homology model for Sortase A was generated usin
Keywords: Staphylococcus pseudintermedius, β-hemolysin, CAMP-test, Listeria monocytogenes, GBS CAMP test is one of the most affordable, easy- (identified with the Liofilchem® O.A. Listeria to-perform methods that may be used in clini- Agar (Figure 2), as well as molecularly) Staphylococcus pseudintermedius is a commensal bacterium of the skin and mucous membrane and is frequently found in companion animals. Carriage of S. pseudintermedius often reaches >80% in some. . Reliable identification of SIG members is critical to establish correct antimicrobial treatments. However, information on the molecular epidemiology and antimicrobial resistance patterns of. Staphylococcus pseudintermedius is an important pathogen in dogs that occasionally causes infections in humans as an opportunistic pathogen of elderly and immunocompromised people. This study compared the genomic relatedness and antimicrobial resistance genes using genome-wide association study (GWAS) to examine host association of canine and human S. pseudintermedius isolates. Canine (n = 25. Identification of a predominant multilocus sequence type, pulsed-field gel electrophoresis cluster, and novel staphylococcal chromosomal cassette in clinical isolates of mecA-containing, methicillin-resistant Staphylococcus pseudintermedius
. Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals Staphylococcus can be a very common cause of infection of the skin and ears of all animals and humans.. Staphylococcus is divided into coagulase positive and coagulase negative species. Coagulase positive species—S. pseudintermedius, S. aureus, and S. schleiferi subspecies coagulans—are more commonly seen; however, coagulase negative species are identified in infections with growing frequency
Pongidae. Pongidae, or the Pongids, is an obsolete primate taxon containing gorillas, chimpanzees and orangutans. They are sometimes called great apes The major antimicrobial resistance microbiological hazards originating from companion animals that directly or indirectly may cause adverse health effects in humans are MRSA, methicillin-resistant Staphylococcus pseudintermedius, VRE, ESBL- or carbapenemase-producing Enterobacteriaceae and Gram-negative bacteria Staphylococcus aureus and this further complicates their identiﬁca-tion . S. pseudintermedius, and not S. intermedius as previously thought, is the most common CPS species isolated from cats and dogs . Therefore, from a phylogenetic point of view, S. pseudin-termedius is not a new emerging species among dogs, but rather The presented strain Staphylococcus pseudintermedius was isolated from a throat swab of a pet dog owner with pharyngitis 11 years before the first report of this species in the taxonomic literature. ZprÆvy EM (SZÚ, Praha) 2010; 19(3): 65Œ67 Staphylococcus spp. infections. Staphylococcus spp., are Gram-positive bacteria, some of which cause suppurative disease processes in animals and humans. Of the over 40 species recognized at present 9, 15 only four (Staphylococcus aureus, S. epidermidis, S. pseudintermedius and S. hyicus) are significant in livestock.These cause various suppurative infections, especially abscesses, mastitis.
Sthaphylococcus pseudintermedius (S. pseudintermedius) is the main inhabitant of skin and mucosa of dogs, where it represents the major bacterial pathogen causing skin diseases. Firstly described in 2005, S. pseudintermedius is a member of the Staphylococcus intermedius Group (SIG), together with S. intermedius and S. delphini. The recent emergence and rapid dissemination of multidrug. . On the other hand, S. intermedius remains an opportunistic pathogen associated with specific hosts, such as pigeons 4
identification, 88 % of the isolates were classified as SIG (Staphylococcus pseudintermedius Group). At the genotypic identification for classification as Staphylococcus pseudintermedius , this number grows to 91 %, showing a significant correlation with the result of biochemical identification. A significant correlatio Staphylococcus est une bactérie du genre : coques, Gram positifs, coagulase positive pour Staphylococcus aureus, Staphylococcus intermedius, Staphylococcus pseudintermedius, Staphylococcus delphini et certains Staphylococcus schleiferi, négatif pour les autres.. Une vingtaine d'espèces de la famille des staphylocoques sont actuellement identifiées, dont l'espèce principale. Staphylococcus pseudintermedius is an important pathogen of companion animals like dogs and cats. Knowledge of sites of colonization is important to detect carriers and prevent underestimation on its Identification of more virulence factors will provide new tactics t
(2007). Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital. (2010). Molecular analysis of methicillinresistant Staphylococcus pseudintermedius of feline origin from different European countries and North America. (2009). Molecular diagnostic identification of Staphylococcus pseudintermedius. (2008) The first infections of methicillin-resistant Staphylococcus pseudintermedius in humans were recorded in 2006, and is now becoming a concern because of its close similarities to human pathogens in the Staphylococcus intermedius group (SIG). These bacteria have all the properties which a multidrug-resistant Staphylococcus aureus possesses.. The literature was searched using the term.
Podsumowanie Staphylococcus pseudintermedius a pathogen difficult to identify Abstract: Staphylococcus pseudintermedius has recently been described as a member of the Staphylococcus genus. Three closely related staphylococci (Staphylococcus pseudintermedius, Staphylococcus intermedius and Staphylococcus delphini) with very similar phenotypic. Staphylococcus pseudintermedius ON 86 is an aerobe, mesophilic, gram-positive human pathogen that was isolated from lung tissue of cat. coccus-shaped gram-positiv Staphylococcus pseudintermedius typing database. Query a sequence Single sequence Query a single sequence or whole genome assembly to identify allelic matches. Batch sequences Query multiple independent sequences in FASTA format to identify allelic matches. Find alleles By specific criteri Staphylococcus pseudintermedius, a resident of the skin and mucosal surfaces of most healthy dogs, is a Gram-positive nonmotile coccus that grows in aerobic and anaerobic conditions, in which it forms grape-like clusters. Staphylococcus pseudintermedius along with the closely related Staphylococcus intermedius and Staphylococcus delphini. Staphylococcus pseudintermedius is a major opportunistic bacterial pathogen and the leading cause of pyoderma in dogs. In canines it is also often associated with infections of the urinary system and wounds and occasionally infects people. Widespread antimicrobial resistance has made the development of alternative treatments a high priority
Molecular methods were used for the correct identification of pathogenic staphylococci. The prevalence of Staphylococcus aureus was identified in 26/72 (36.1%) dog owners and in 4/61 (6.6%) dogs. Staphylococcus pseudintermedius was isolated from three (4.2%) humans, and from 28 dogs (45.9%). Other coagulase-positive staphylococci species were. DOE PAGES Journal Article: Characterization of a leukocidin identified in Staphylococcus pseudintermedius. Characterization of a leukocidin identified in Staphylococcus pseudintermedius as S. pseudintermedius (identification accuracy, 92%) using the Vitek2 system with gram-positive ID cards (BioMérieux Japan Ltd., Tokyo, Japan). The strains showed white-gray color colonies on sheep-blood agar plates with β-hemolysis, and a Staphylococcus latex agglutination test performed us-ing PS latex (Eiken Chemical Co., Ltd, Tokyo. The species Staphylococcus pseudintermedius was originally described and validly published by Devriese et al. 2005. Citation When referring to this Abstract, please use its Digital Object Identifier and cite NamesforLife
Staphylococcus pseudintermedius is a leading aetiologic agent of pyoderma and other body tissue infections in dogs and cats. In recent years, an increased prevalence of methicillin-resistant S. pseudintermedius (MRSP) has been reported. Isolation of MRSP in serious infections poses a major therapeutic challenge as strains are often resistant to all forms of systemic antibiotic used to treat S. Identification and antimicrobial resistance profile of Staphylococcus pseudintermedius Isolated from canine pyoderma. 2012. 67 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2012 We report the antibiotic treatments administered to a female dog with mastitis and successive pyoderma. Microbiological investigations allowed the identification of Staphylococcus pseudintermedius after 54 days of various antibiotic treatments. The isolate carried the mecA gene and was resistant to 9 of 15 tested antibiotics Cefoxitin breakpoint for Staphylococcus pseudintermedius 965 practices in Florida, Oregon, and the surrounding states of North Carolina and Kentucky. Members of the Staphylococcus intermedius group (SIG) consist of the closely related species S. intermedius, S. pseud- intermedius, and Staphylococcus delphini. 10 Identification of S. pseudintermedius was performed as previously describe
The occurrence of antimicrobial resistance in Staphylococcus pseudintermedius from skin and ear infections in a representative sample of dogs unexposed to antimicrobial treatment before sampling was examined. The obtained isolates were further examined for genetic polymorphism and genetic background of resistance Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini. Microorganisms . Basel: MDPI, 2020, roč. 8, č. 2, s. 204-1-204-18, 18 s Staphylococcus pseudintermedius is an opportunistic and emerging zoonotic pathogen that primarily colonises the skin of dogs. Many common variants are methicillin resistant (MRSP) or multidrug resistant (MDR), and drug resistance is increasingly reported across the globe. In New Zealand, MRSP isolation remains rare in clinics. To pre-emptively inform diagnostic and antimicrobial stewardship. Identification of the virulence gene regulatory network, and its component virulence genes, may lead to the development of a staphylococcus-specific treatment option. This would provide additional therapeutic strategies for the treatment of canine pyoderma and other S. pseudintermedius infections Infections by Staphyloccocus pseudintermedius in the skin of dogs have been object of studies, since such microorganisms often present multiple resistance to antibiotics. This study aimed to identify and evaluate the antimicrobial susceptibility of Staphylococcus pseudintermedius (SP) strains isolated from dogs with otitis
Staphylococcus pseudintermedius is a common opportunistic pathogen in domestic canines and felines. This pathogen is known to cause skin/soft tissue and post-operative wound infections .Human infection with S. pseudintermedius is increasingly being demonstrated and is now considered an emerging pathogen; displaying the ability to colonize human skin and mucous membranes Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an increasingly prevalent pathogen in veterinary medicine. This study examined the molecular epidemiology of clinical MRSP isolated from Australian animals. Clinical staphylococci submitted to all Australian veterinary diagnostic laboratories were collected during 2013 and identified using traditional phenotypic tests and matrix. S. pseudintermedius is an important opportunistic pathogen of companion animals. It is the most frequent Staphylococcus isolates collected from infected dogs. Since 2006 there has been a significant emergence of MRSP .Many of MRSP isolates from Europe and USA (about 54%) were classified as ST71-SCCmec II, III or NT (NT: nontypable) [11, 12].The MRSP recovered here from dog (isolate BMBSP02. Iyori K, Hisatsune J, Kawakami T, Shibata S, Murayama N, Ide K, Nagata M, Fukata T, Iwasaki T, Oshima K, Hattori M, Sugai M, Nishifuji K (2010) Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs. FEMS Microbiol Lett 312: 169-175
The workflow of these experiments referred to a previously described method, with some modifications . Briefly, overnight cultures of the S. chromogenes isolate SC10, Staphylococcus agnetis isolate 12-4S-3, S. aureus isolate 12-1N-1, Staphylococcus haemolyticus isolate X2-2N-1, Staphylococcus warneri isolate X1-2S-2, Staphylococcus pseudintermedius isolate 29HD2S-5 and Staphylococcus.